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101Bio P528 Endosome Isolation Kit, 20 rxn
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Endosome Isolation Kit
Name
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Endosome Isolation Kit
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Cat. #
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P528
Step 1
Step 2
Step 1
Step 2
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Application
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Rapid and simple endosome isolation using spin-column, requiring smaller amount of cells or tissues.
This product is for research use only.
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Product Size
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20 reactions |
Description
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Early endosomes (EE) provide the starting point for late endosome maturation. The EEs are mainly derived from primary endocytic vesicles that fuse with each other. EEs receive endocytic cargos not only through the clathrin-mediated pathway but several other pathways. In addition to their roles in normal cell physiology, endocytic processes play a key role in many diseases such as Alzheimer’s disease and inherited lysosomal storage diseases. Traditional methods for isolating endosomes are based on density gradient ultracentrifugation. The protocol requires large amount of starting material and the methods are tedious and time consuming.
101Bio Endosome Isolation Kit provides a spin-column based novel endosome isolation technology which is rapid and simple. It only requires smaller amount of cultured cells or milligram amounts of tissues. This kit can precipitate and significantly enrich early endosomes from cultured cells or tissues. The availability of the kit should facilitate the research in the field.
- Fast - 20 minutes hands-on
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Shipping / Storage
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Ship at ambient temperature, store this kit at room temperature except Buffer A at 4oC |
Shelf Life
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12 months |
Manual (protocol)
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Components
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| Components | Amount | Storage |
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| Buffer A |
15 mL |
4oC |
| Buffer B |
15 mL |
4oC |
| Plastic rods |
2 |
Room temperature |
| Filter cartridges with collection tubes |
20 |
Room temperature |
| Tissue dissociation beads |
2.5 g |
Room temperature |
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Remarks
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- 1. Read the entire procedures carefully. Chill protein extraction filter cartridge with collection tube on ice prior to use.
- 2. All centrifugation steps should be performed at 4oC in a cold room or in a refrigerated microfuge.
- 3. To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use. The use of protease inhibitor cocktails is optional.
- 4. It is recommended to use BCA Protein Assay Kit for determination of protein concentration.
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Protein Analysis Reagents
Step 1
Step 2
Step 1
Step 2
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