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101Bio P502 Total Protein Extraction Kit (Animal Tissues), 20 rxn


Supplier: 101Bio.com

Price: SGD$366.92
  Total Protein Extraction Kit    (Animal Tissues)
Warning:   Use RIPA buffer, you get biased data (Western Blot, IP, ELISA, etc.) due to uneven protein loss..  
Replace it by our P502 which can extract full spectrum total protein in 3 minutes.

Name

3-min. Total Protein Extraction Kit (Animal Tissues)

Cat. #

P502S
 
(  5 reactions)   

P502
(20 reactions)   

P502L
     

Application

Extract full spectrum total protein from animal (human) tissues in 3 minutes.   This product is for research use only.

Description

RIPA buffer has been used as a “gold standard” for total protein extraction for 20 years. In fact, it fails to extract true total protein.  Due to its method defect, RIPA produce biased data (Western Blot, IP, ELISA, etc.) caused by uneven protein loss and leads to wrong interpretations.  Recently, it has been reported by more and more researchers that the data from RIPA are questionable.

Our P502 is an ultimate solution which can extract ture total protein (full spectrum protein profile).

The total protein extracted by our P502 can be used in all Western Blot, IP and co-IP assay.

  • Fast - 1 to 3 minutes
  • Full spectrum protein profile
  • For all animal tissues: mammalian, insets and other animal tissues
  • Flexible extraction volume: 20 µl to 500 µl (useful for small amount of sample)
  • High yield and high concentration - 2 ~ 8 mg/ml
  • Ideal for small volume of sample - can handle 15 ~ 20 mg of tissues

Shipping / Storage

Ship at room temperature.  Store "Native lysis buffer" at 4℃, and the rest at room temperature. (Do not freeze.)

Shelf Life

12 months

Manual (protocol)

  
  Total Protein Extraction Kit (Animal Tissues)

Components

P502 (20 rxn) ComponentsAmountStorage
Denaturing lysis buffer 10 mL Room temperature
Native lysis buffer 10 mL 4℃
Filter Cartridges 20 Room temperature
Collection tubes with cap 20 Room temperature
Plastic rods (reusable) 2 Room temperature

Remarks

  • Protein profiles extracted by denaturing and native lysis buffer are not identical. For a given application one lysis buffer might be superior to the other. This kit provides both denaturing and native lysis buffers for users to test and select the best one for a specific application.
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to lysis buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.
protein extraction kit for animal tissues
                   
Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.    Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, gold fish muscle and 5, mouse liver