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101Bio P505L Detergent-free Total Protein Extraction Kit (Animal cells), 50 rxn


Supplier: 101Bio.com

Price: SGD$690.00
Detergent-free Total Protein Extraction Kit  &nbsp &nbsp (Animal cells)

Name

Detergent-free Total Protein Extraction Kit (Animal cells)

Cat. #

P505S
 

Application

Rapidly extract detergent-free total proteins from cultured cells (mammalian cells, insect cells and other cultured cells) in less than 5 minute.

The extracted protein can be used for proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications.

This product is for research use only.

Description

This kit is for fast extraction of detergent-free total proteins from animal cells.  The extraction does not contain any detergent and EDTA.  The extraction volume is flexible, from 20 µl to 500 µl, which is useful for limiting number of cells.

  • Fast - less than 5 minutes
  • High yield and high concentration - 1 ~ 5 mg/ml
  • Ideal for small volume of sample - can handle 0.5 ~ 3 million cells

Shipping / Storage

Ship at room temperature.  Store at 4℃ (Do not freeze.)

Shelf Life

12 months

Manual (protocol)

  
  Detergent-free Total Protein Extraction Kit (Animal cells)

Components

ComponentsAmountStorage
Buffer A 6 mL 4℃
Buffer B 6 mL 4℃
Filter Cartridges 20 Room temperature
Collection tubes with cap 20 Room temperature

Remarks

  • Detergent-free total proteins can be extracted from cells in less than 5 minutes.
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to Buffer A is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use.
Detergent-free Total Protein Extraction Kit for Animal cells