Browse Categories

101Bio P507L Mitochondria Isolation Kit (Mammalian cells / tissues), 50 rxn


Supplier: 101Bio.com

Price: SGD$851.54
Mitochondria Isolation Kit  &nbsp(Mammalian cells / tissues)

Name

Mitochondria Isolation Kit (Mammalian cells / tissues)

Cat. #

P507L
 
 

Application

Rapidly isolate intact mitochondria from mammalian cells or tissues in less than 30 minute.

This product is for research use only.

Product Size

P507L: 50 rxn

Description

This kit is for fast isolation of intact mitochondria from animal tissues.  The isolated mitochondria does not contain any detergent and EDTA.  

  • Fast - less than 30 minutes
  • High yield and high concentration - 1 ~ 3 mg/ml
  • Ideal for small volume of sample - can handle 5 - 40 million cells

Shipping / Storage

Ship at room temperature.  Store at -20℃

Shelf Life

12 months

Manual (protocol)

  
  Mitochondria Isolation Kit  (Mammalian cells / tissues)

Components

ComponentsAmountStorage
Buffer A 15 mL -20℃
Buffer B 30 mL -20℃
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 4 Room temperature
Tissue dissociation beads 4g Room temperature

Remarks

  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to Buffer A is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use.