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101Bio P519-20 Protein Extraction Kit from Gel Slices, 20 rxn
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 Protein Extraction Kit from Gel Slices
Name
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8-min. Protein Extraction Kit from Gel Slices
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Cat. #
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P519-20
Step 1
Step 2
Step 1
Step 2
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Application
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8 minutes gel slice protein recovery at high yield.
Extracted proteins can be used for MALD-MS analysis, immunization of animals, protein-protein interaction and protein-nucleic acid interaction studies etc.
This product is for research use only.
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Product Size
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P519-20: 20 reactions; P519-80: 80 reactions |
Description
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This kit is for fast extraction of proteins from gel slices.
- Fast - 8 minutes
- No overnight waiting
- No need for special device
- High efficient for 10~200 kDa protein
- high concentration - 10 ~ 200 uL final volume - Multiple gel pieces can be processed in one tube.
- Can be detergent-free
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Shipping / Storage
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Ship and store at room temperature. |
Shelf Life
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12 months |
Manual (protocol)
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8-min. Protein Extraction Kit from Gel Slices |
Components
(Cat.#: P519-20)
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| Components | Amount | Storage |
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| Filter Cartridges |
20 |
Room temperature |
| Collection tubes with cap |
20 |
Room temperature |
| Microfuge tubes (200 uL) |
20 |
Room temperature |
| Extraction powder |
2g |
Room temperature |
| Micro pestle |
4 |
Room temperature |
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Remarks
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- The choice of elution buffer depends on how gel is stained and the downstream applications.
- If the stain contains fixing agent such as methanol and acetic acid, an elution buffer containing 0.1-0.5% SDS or acid labile surfactants is recommended.
- If the gel is un-stained or negatively stained the protein can be eluted with ddH2O or an elution buffer containing formic acid/water/2-propanol (1:3:2 v/v/v).
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Protein Analysis Reagents
Step 1
Step 2
Step 1
Step 2
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