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101Bio P510L Plant Tissue Detergent-free Total Protein Extraction Kit, 50 rxn


Supplier: 101Bio.com

Price: SGD$851.54
Plant Tissue Detergent-free Total Protein Extraction Kit

Name

8-min. Plant tissue Detergent-free Total Protein Extraction Kit

Cat. #

P510L
     
 

Application

Extract detergent-free total protein from plant tissues within 8 minutes.

Can handle frozen or fresh leaves, seeds, soft stem and roots, etc.

For proteomics(LC/MS), ELISA, SDS-PAGE, immunoblottings, IP,  etc.

This product is for research use only.

Product Size

P510L: 50 rxn

Description

This kit is for fast extraction of water soluble proteins from plant tissues.

   

The protein extraction does not contain any detergent and organic solvent.

  • Fast - 5 to 8 minutes
  • High yield and high concentration - 1 ~ 5 mg/ml
  • Ideal for small volume of sample - extraction volume can be 50 ~ 500 uL

Shipping / Storage

Ship at room temperature.  Store at 4℃. (Do not freeze.)

Shelf Life

12 months

Manual (protocol)

  
  8-min Plant Tissue Detergent-free Total Protein Extraction Kit

Components

ComponentsAmountStorage
Detergent-free lysis buffer 25 mL 4℃
Filter Cartridges 50 Room temperature
Collection tubes with cap 50 Room temperature
Plastic rods 4 Room temperature

Remarks

  • Total proteins can be extracted from plant tissue in 5-8 min
  • Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to lysis buffer is recommended.
  • For determination of protein concentration, BCA kit (Pierce) is recommended.
  • To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to lysis buffer prior to use.