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101Bio W0500-50 Pure Plasmid Miniprep Kit, 50 rxn


Supplier: 101Bio.com

Price: SGD$136.15
Pure Plasmid Miniprep Kit

Name

Pure Plasmid Miniprep Kit

Cat. #

W0500-50
(  50 reactions)

W0500-200
(200 reactions)

Application

SampleYieldElutionMethodDNA Quality
1 ~ 5 mL E. Coli 3 ~ 30 μg plasmid DNA 50 ~ 100 μL Spin Column Transfection, PCR, Enzyme digestion, Sequencing
This product is for research use only.

Description and features

This kit is based on a modified alkaline lysis method, with improved reagent formulation and silica membrane, which has high adsorption efficiency of the plasmid DNA. The proteins, genomes, RNA and other impurities can be removed after washing and an elution.

3 ~ 30 μg of plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit.

The extracted DNA can be directly used for PCR, restriction enzyme digestion, sequencing, and transfection.   No need to precipitate, concentrate or desalt.

Shipping / Storage

Ship at room temperature.  Store RNase A or RNase A added buffer P1 at 4oC

Shelf Life

12 months

Manual (protocol)

  
  Pure Plasmid Miniprep Kit

General Procedure

Pure Plasmid DNA Miniprep Kit, Plasmid DNA purification Kit

Components

ComponentsAmountStorage
W0500-50 W0500-200
Buffer P1 15 mL 60 mL Room temperature; store at 4oC if RNase A is added.
Buffer P2 15 mL 60 mL Room temperature
Buffer N3 20 mL 80 mL Room temperature
Buffer PB 30 mL 120 mL Room temperature
Buffer PW ( concentrate ) 10 mL 40 mL Room temperature
Buffer EB 10 mL 30 mL Room temperature
RNase A  ( 10 mg/mL ) 150 μL 600 μL 4oC
Spin Column 50 200 Room temperature
2 mL Collection Tube 50 200 Room temperature