Browse Categories

101Bio W0636-100 100 bp DNA Ladder (100 bp - 1,500 bp, with loading dye), 100 rxn


Supplier: 101Bio.com

Price: SGD$459.23
100 bp DNA Ladder (100 bp ~ 1,500 bp, with loading dye)

Name

100 bp DNA Ladder (100 bp ~ 1,500 bp, with loading dye)

Cat. #

W0636-100
(   500 µL, 100 rxn)

Application

100 bp DNA Ladder contains 9 DNA fragments, 1,500 bp, 1,000 bp, 800 bp, 600 bp, 500 bp, 400 bp, 300 bp, 200 bp, and 100 bp.   This is a DNA solution with 1 × Loading Buffer.  Directly load 5 μL 100 bp DNA Ladder to electrophoresis gel when using.   The 500 bp band is ~ 150 ng / 5 µL and much brighter than the other bands as a visible reference indicator. The other DNA bands are ~ 50 ng / 5 µL.

This product is for research use only.

How to use

Load 5 µL of 100 bp DNA Ladder to the agarose gel (If the sample hole is larger than 6mm, increase the sample volume proportionally) and run electrophoresis.

Shipping / Storage

Store at -20℃ for up to 2 year.  For frequent use, store at 4℃ for up to 6 months. Avoid freeze-thaw cycles.

Manual (protocol)

  
  100 bp DNA Ladder                       

Result

100 bp DNA Ladder, 100 bp ~ 1,500 bp DNA ladder

The gel image shown is 5 µL of 100 bp DNA Ladder separated by 1% agarose gel.

Remark

  • Do not heat before loading.
  • Please use fresh electrophoresis buffer and agarose gel for the best result.
  • High quality agarose should be used to avoid effecting the DNA bands and the recommended agarose gel concentration is 1% ~ 3%, recommended voltage is 4 ~ 8 v / cm.
  • The separation performance of DNA fragments in agarose gel is related to gel concentration. The higher concentration gel separates shorter DNA fragments better.  The lower concentration gel separates longer DNA segments better.
  • This product is not for diagnostic use. The safety and efficacy of this product in diagnostics or other clinical uses have not been established.