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101Bio W0655-5 2x GoldStar Best Master Mix (with dye), 5 ml


Supplier: 101Bio.com

Price: SGD$530.77
2x GoldStar Best Master Mix (with dye)

Name

2x GoldStar Best Master Mix (with dye)

Cat. #

W0655-1
(1 mL)

W0655-5
(5 mL)
    

Application

  • Routine PCR
  • High fidelity gene cloning
This product is for research use only.

Specifications

Polymerase: GoldStar Best Taq
Hot Start: Built-In Hot Start
Fidelity: High fidelity
GC-Rich PCR Performance: High
Amplification Range: 6 kb
Exonuclease Activity: 5´→3´ and 3´→5´
Reaction Speed: 1 kb / 60 seconds
Reaction Format: Master Mix
Product Overhang: 3'-A

Description and features

2x GoldStar Best Master Mix is a convenient premixed 2x concentrated solution for PCR which include  GoldStar Best DNA Polymerase, PCR Buffer, Mg2+, dNTPs, PCR Stabilizer and PCR Intensifier.

The polymerase has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity.  It also possesses 3´→5´ exonuclease (proofreading) activity.  The polymerase has a higher amplification efficiency and lower mispairing rate than routine Taq DNA polymerase.

The GoldStar Best is a high reliable hotstart polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton.  This prevents the formation of misprimed products and primer dimers at ambient temperatures.  GoldStar Best Polymerase is activated by a 10 minutes, 95°C incubation.   Optimized buffer system promotes the amplification of target fragment with high fidelity, high specificity, high amplification efficiency and high sensitivity.

GoldStar Best DNA Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning.

The amplification range of Es Taq is ~ 6 kb.

The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.

Shipping / Storage

Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. Stored at 4℃ for up to 3 months.

Quality control

This product is tested for no exogenous nuclease activity;  no host DNA contamination tested (by PCR);  able to amplify single copy gene from multiple genomes; and no significant enzyme activity decrease after storing at 2 ~ 8oC for 3 months.

Manual (protocol)

  
  2x GoldStar Best Master Mix

Components

 
ComponentsAmount
W0655-1 W0655-5
2x GoldStar Best Master Mix 1 mL 1 mL x 5
RNase-Free Water 1 mL 5 mL

PCR reaction system

2x Es Taq Master Mix, convenient, save time and reliable

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background.

PCR reaction conditions

2x Es Taq Master Mix, convenient, save time and reliable

Note:

  • The recommended annealing temperature is about 5℃ below Tm of primers. If extra bands are observed, higher annealing temperatures should be considered. The absence of product indicates the need for a lower annealing temperature.
  • PCR extension time is depended on the size of target gene sequence.  GoldStar Best DNA polymerase is approximately 1 kb DNA / 60 seconds.
  • The number of PCR cycles will basically depend on the downstream application of the PCR product.

PCR result examination

This Master Mix contains dye for electrophoresis.  After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis.  No need to add loading buffer.