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101Bio W0690-1 2x Es Taq Master Mix (with dye), 1 ml


Supplier: 101Bio.com

Price: SGD$159.23
2x Es Taq Master Mix (with dye)

Name

2x Es Taq Master Mix (with dye)

Cat. #

W0690-1
(1 mL)

W0690-5
(5 mL)
    

Application

  • Routine PCR
  • T/A cloning
This product is for research use only.

Specifications

Polymerase: Es Taq
Hot Start: N/A
Fidelity: High fidelity
GC-Rich PCR Performance: High
Amplification Range: 6 kb
Exonuclease Activity: 5´→3´
Reaction Speed: 1 kb / 30 seconds
Reaction Format: Master Mix
Product Overhang: 3'-A

Description and features

This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer.  The concentration is 2x.

Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity.  The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase.  Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning.   The amplification range of Es Taq is ~ 6 kb.

This unique Master Mix recipe makes the system very reliable.  More than 98% of PCR reaction can get successful amplification during the first try.   It also works well on complicate templates.

The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.

Shipping / Storage

Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. Stored at 4℃ for up to 3 months.

Quality control

This product is tested for no exogenous nuclease activity;  no host DNA contamination tested (by PCR);  able to amplify single copy gene from multiple genomes; and no significant enzyme activity decrease after storing at 2 ~ 8oC for 3 months.

Manual (protocol)

  
  2x Es Taq Master Mix

Components

 
ComponentsAmount
W0690-1 W0690-5
2x Es Taq Master Mix 1 mL 1 mL x 5
RNase-Free Water 1 mL 5 mL

PCR reaction system

2x Es Taq Master Mix, convenient, save time and reliable

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background.

PCR reaction conditions

2x Es Taq Master Mix, convenient, save time and reliable

Note:

  • The recommended annealing temperature is about 5℃ below Tm of primers. If extra bands are observed, higher annealing temperatures should be considered. The absence of product indicates the need for a lower annealing temperature.
  • PCR extension time is depended on the size of target gene sequence.  Es Taq DNA polymerase is approximately 1 kb DNA / 30 seconds.
  • The number of PCR cycles will basically depend on the downstream application of the PCR product.

PCR result examination

This Master Mix contains dye for electrophoresis.  After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis.  No need to add loading buffer.