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101Bio W109-100 50/100 bp DNA Ladder (50 bp - 1,500 bp, with loading dye), 100 rxn


Supplier: 101Bio.com

Price: SGD$459.23
50/100 bp DNA Ladder (50 bp ~ 1,500 bp, with loading dye)

Name

50/100 bp DNA Ladder (50 bp ~ 1,500 bp, with loading dye)

Cat. #

W109-100
(   600 µL, 100 rxn)
    

Application

50/100bp DNA Ladder has 11 unique DNA bands ranging from 50 ~ 1500 base pairs (bp). It is specially designed for visualizing DNA bands in the x50-bp-positions, such as 150 bp, 250 bp, 350 bp, etc.   It is helpful when use this ladder side by side with the 100 bp DNA Ladder (Cat. #: W107-100) for more accurate size estimates of DNA fragments.   The 550 bp band is brighter than other bands to serve as an internal reference.  Concentration: 750 ng / 6 μL

This product is for research use only.

How to use

Load 6 µL of 50/100 bp DNA Ladder to the agarose gel (If the sample hole is larger than 6mm, increase the sample volume proportionally) and run electrophoresis.

Shipping / Storage

Store at -20℃ for up to 2 year.  For frequent use, store at 4℃ for up to 6 months. Avoid freeze-thaw cycles.

Manual (protocol)

  
  50/100 bp DNA Ladder                       

Result

50/100 bp DNA Ladder, 50 bp ~ 1,500 bp DNA ladder

The gel image shown is 6 µL of 50/100 bp DNA Ladder separated by 1.5% agarose gel.

Remark

  • Do not heat before loading.
  • Please use fresh electrophoresis buffer and agarose gel for the best result.
  • High quality agarose should be used to avoid effecting the DNA bands and the recommended agarose gel concentration is 1% ~ 3%, recommended voltage is 4 ~ 8 v / cm.
  • The separation performance of DNA fragments in agarose gel is related to gel concentration. The higher concentration gel separates shorter DNA fragments better.  The lower concentration gel separates longer DNA segments better.
  • This product is not for diagnostic use. The safety and efficacy of this product in diagnostics or other clinical uses have not been established.