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101Bio W2569-25 HiFiScript 1st Strand cDNA Synthesis Kit, 25 rxn


Supplier: 101Bio.com

Price: SGD$228.46
HiFiScript 1st Strand cDNA Synthesis Kit

Name

HiFiScript 1st Strand cDNA Synthesis Kit

Cat. #

W2569-25
( 25 rxn)

W2569-100
(100 rxn)
    

Application

  • Reverse transcription, 1st strand cDNA synthesis
  • cDNA library construction
This product is for research use only.

Specifications

Optimal Reaction Temperature: 42℃
Final cDNA size: Up to 12 kb
Reaction time: 30 ~ 50 min.
Reverse Transcriptase: HiFiScript
RNase H Activity: Reduced
Sensitivity: High
cDNA yield: High

Description and features

HiFiScript 1st Strand cDNA Synthesis Kit has been designed for the first-strand cDNA synthesis kit in a two-step RT-PCR experiments.  HiFiScript reverse transcriptase in the kit is a new recombinant Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) with novel mutations,  which endorse HiFiScript the following features:

  • High transcriptional efficiency – higher yields of cDNA
  • High sensitivity – pg level total RNA and/or mRNA can be used to synthesis first strand cDNA.
  • RNase H activity is removed – improved the elongation ability of the enzyme, and increased affinity of transcriptase to RNA template.   Generate first strand cDNA up to 12 kb.

The optimized RT Buffer make the reverse transcriptase a wider range of applications, and with high compatibility for subsequent PCR and quantitative PCR experiments.

Shipping / Storage

Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. /td>

Manual (protocol)

  
  HiFiScript 1st Strand cDNA Synthesis Kit

Components

tr>dNTP Mix, 2.5 mM Each120 μL500 μL 
ComponentsAmount
W2569-25 W2569-100
HiFiScript, reverse transcriptase, 200 U/μL 25 μL 100 μL
5X RT Buffer 120 μL 500 μL
Primer Mix 60 μL 240 μL
DTT, 0.1 M 60 μL 240 μL
RNase-Free Water 1 mL 5 mL

RT reaction system

HiFiScript 1st Strand cDNA Synthesis Kit

Note: If the starting RNA is less than 50 ng, RNase Inhibitor (RNasin, Cat.#: W0596) should be added.