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101Bio W2581-10 Endotoxin-Free Plasmid Midiprep Kit, 10 rxn


Supplier: 101Bio.com

Price: SGD$228.46
Endotoxin-Free Plasmid Midiprep Kit

Name

Endotoxin-Free Plasmid Midiprep Kit

Cat. #

W2581-10
(10 reactions)
    

Application

SampleYieldElutionMethodDNA Quality
15~50mL E. Coli 25~250 μg EndoFree plasmid DNA 0.5 ~ 1.0 mL Spin Column Transfection, PCR, Enzyme digestion, Sequencing
This product is for research use only.

Description and features

Eukaryotic cells are very sensitive to endotoxin, a common contaminant in the plasmid extraction, and the transfection efficiency of the cells will greatly reduce if the plasmid contains endotoxin. This kit can efficiently extract endotoxin-free plasmid in a fast and simple way. The endotoxin, genomic DNA, RNA, protein and other impurities can be largely removed.

25 ~250 μg transfection-grade plasmid DNA can be purified from 15 ~ 50 mL overnight culture of E. Coli in LB medium with this kit within 50 minutes.

The extracted DNA can be directly used for Transfection PCR, restriction enzyme digestion, sequencing, etc.   No need to precipitate, concentrate or desalt.

Shipping / Storage

Ship at room temperature.  Store RNase A or RNase A added Buffer P1 at 4oC

Shelf Life

12 months

Manual (protocol)

  
  Endotoxin-Free Plasmid Midiprep Kit

General Procedure

Endotoxin-Free Plasmid DNA Midiprep Kit, EndoFree Plasmid DNA purification Kit

Components

ComponentsAmountStorage
Buffer P1 30 mL Room temperature; store at 4oC if RNase A is added.
Buffer P2 30 mL Room temperature
Buffer E3 30 mL Room temperature
Buffer PS 12 mL Room temperature
Buffer PW ( concentrate ) 15 mL Room temperature
Endotoxin-Free Buffer EB 30 mL Room temperature
RNase A  ( 10 mg/mL ) 500 μL 4oC
Endotoxin-Remover Filter 10 Room temperatureC
Plunger 10 Room temperatureC
Spin Column (Midi) 10 Room temperatureC
Collection Tube 20 Room temperatureC