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101Bio W2601-5 Ultra SYBR Green qPCR Master Mix (2X, with ROX I), 5 ml


Supplier: 101Bio.com

Price: SGD$690.00
Ultra SYBR Green qPCR Master Mix (2X, with ROX I)

Name

Ultra SYBR Green qPCR Master Mix (2X, with ROX I)

Cat. #

W2601-1
(1 mL)

W2601-5
(5 mL)
    

Application

  • Gene expression analysis
  • Microarray result validation
This product is for research use only.

Specifications

Polymerase: GoldStar Best Taq
Hot Start: Built-In Hot Start
Detection Method: SYBR Green
GC-Rich PCR Performance: High
Sample Type: Genomic DNA, cDNA
Platform: ABI Prism7500/7500 Fast,  Stratagene Mx3000/Mx3005P,  Corbett Rotor Gene 3000
Reaction Speed: 1 kb / 60 seconds
Reaction Format: 2X Master Mix

Description and features

UltraSYBR Mixture (With ROX) provides a convenient real-time PCR specific reagent by intercalater method using SYBR Green I for detection.  It is a 2x concentrated premix of GoldStar Taq DNA polymerase, dNTPs, Mg2+, SYBR Green I dye (detection), ROX reference dye and PCR buffer components.  The product utilizes an enzyme for hot start, GoldStar Taq DNA polymerase, which confers a significant reduction in non-specific PCR amplification.  As the enzyme buffer system is optimized for real time PCR, the product offers high amplification efficiency and high detection sensitivity in real-time PCR.

In light of the fact that the qPCR instruments can vary from user to user, Cowin offers the UltraSYBR Mixture in a range of formulations, each of which has been carefully optimized to confer the best performance according to the make and model of a qPCR machine.  Please use the following table as a guide for selecting the UltraSYBR Mixture that will be most compatible with your choice of a particular instrument/model.

Shipping / Storage

Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. Stored at 4℃ if it is frequent used within short time.

Key features

  • Easy-to-use 2X premix reagent including SYBR Green I
  • Hot start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
  • Accurate detection and quantification of target gene through real time PCR.

Manual (protocol)

  
  Ultra SYBR Green qPCR Master Mix (2X, with ROX I)

Components

 
ComponentsAmount
W2601-1 W2601-5
Ultra SYBR Green qPCR Master Mix (2X, with ROX I) 1 mL 1 mL x 5
RNase-Free Water 1 mL 5 mL

PCR reaction system

Ultra SYBR Green qPCR Master Mix (2X, with ROX I)

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer.  A final primer concentration of 0.2 µM is likely to yield good results. Adjust the primer concentraction as necessary.